The study was carried out to investigate the various estrus synchronization protocols for induction of estrus in pubertal anestrous Gir heifers. A total of 32 pubertal heifers that failed to exhibit estrus were selected and randomly divided into four groups of 08 heifers each. Three estrus induction/synchronization protocols viz. CIDR +PG (G1), Ovsynch (G2) and Cosynch (G3) along with one control group (G4) were evaluated for efficacy of estrus induction and synchronization. Estrus induction responses of 87.50%, 75.00%, and 75.00 % were observed in G1, G2, and G3, respectively; whereas, subsequent fertility response in terms of conception rates were obtained as 50.00 %, 37.50 % and 25.00 % in G1, G2, and G3, respectively. However, in control group (G4) no heifer showed sign of estrus during the experiment period. It can be concluded that induction of estrus and improvement in conception is possible with the use of different

hormone protocols, viz. CIDR+PG, Ovsynch and Cosynch in pubertal anestrous Gir heifer. For treating pubertal anoestrus as well as for subsequent fertility, CIDR+PG was found to be most effective out of the three hormonal protocols followed by ovsynch and cosynch.